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Energy transfer studies in hematin-porphyrin monomer complexes: a size selective fluorescent excitation spectrum is observed.
A combination of resonance Raman and pulse radiolysis (PR) spectroscopic techniques has been used to characterize the excited state properties of hematin, meso- and pheomelanins, i.e. the chromophore of the eumelanin pigment hematin. The microenvironments of the axial ligands at the hematin iron(II) center have been studied in these compounds. The findings indicate a size selective excitation of the hematin chromophore by the porphyrin core.Efficient phosphonate phosphatase systems in the genome of Pythium oligandrum, Pythium irregulare, and Pythium insidiosum.
Phosphate metabolism is a critical process in the life cycle of pathogenic oomycetes, and genes encoding enzymes involved in phosphonate metabolism are strongly differentially expressed during infection. Here, we identified and characterized genes encoding two phosphonate phosphatases, PIP and PHOS, which are present in two closely related species of Pythium (P. irregulare and P. oligandrum) and in a pathogenic strain of P. insidiosum. The PIP and PHOS genes were strongly expressed in haustoria of Pythium species that colonize the host, and P. insidiosum, which also forms haustoria. The amino acid sequence of PIP from P. insidiosum was similar to that of PIPs from P. irregulare and P. oligandrum, but that of PHOS was quite distinct, suggesting that these phosphonate phosphatase genes are orthologs and underwent a gene duplication event during evolution. We also found that PIP and PHOS proteins are targeted to the plant cell wall in spite of their ability to hydrolyze the ubiquitous and cytotoxic phosphorus-containing compound H(3)PO(4). PIP and PHOS proteins of Pythium species hydrolyzed phosphonates with the phosphate group bound to carbon-10, and both enzymes were mainly functional in the cytoplasm of plant cells. The expression of these genes in host cells was regulated by both phosphate and carbon-catabolite repression, suggesting that these phosphonate phosphatase genes are regulated by phosphate starvation and/or two-

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LANSING, MI – Gov. Gretchen Whitmer said Friday she’d consider a proposal to allow voters to decide on whether to legalize marijuana through a ballot initiative.

Whitmer’s remark comes a day after dispensary owners and advocates argued a voter referendum could deflate support for the state’s medical marijuana program. Michigan’s medical marijuana program is one of the largest in the nation, in some places covering 10 times the number of patients and costing the state about $1.1 million per week in sales taxes and fees.

“It’s an area we’ve really looked at,” Whitmer said during a tour of Fairlane Village retirement community in Lansing. “Let’s think about that for a little bit.”

“We would be open to reviewing that,” she said, adding that she and Attorney General Dana Nessel met with the Voter Coalition on Medical Marijuana last year to discuss the proposal.

“It’s something we talked about,” Whitmer said, adding that she’d like to see a proposal presented to voters for a November ballot question.

But she stopped short of saying she’s willing to go on the ballot herself to support the proposal.

“I would listen to a plan,” she said. “I think that would be a conversation that would involve all of us.”

Whitmer stopped short of taking the position of medical marijuana advocate John Truscott, who has proposed a ballot measure for 2018. The Republican Truscott has said that Michigan’s medical marijuana program has failed its patients and should be shut down.

Whitmer said legalizing pot for recreational use could generate tax revenue for the state.

The governor also indicated she’d been working with businesses to come up with a proposal for a legal marijuana system that could be created. Whitmer said she wants to protect the industry from corrupting influences.

“You need to be able to trust that you’re going to have the businesses that are looking after Michigan’s interest,” she said.

On the tour, Whitmer stopped short of endorsing the legalization of marijuana for recreational use, saying “we don’t know enough yet,” but said she did not oppose it.

“That’s something that we’d have to look at,” she said. “We don’t know enough yet, but if I had my ideal world, I’d prefer to see it done on a medical rather than a broader, recreationally basis.”Q:

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