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The binding site for dihydropyridine (DHPR) receptor antagonists in the apical membrane of the intestinal epithelial cells has been identified. In the presence of the antagonist D600 [( 3H]nicardipine) the apical membrane of polarized intestinal epithelial cells selectively binds large amounts of this ligand. In contrast, the basal membrane of the intestinal epithelium is only poorly accessible for nicardipine. Similar findings are obtained with the DHPR antagonist niladipine. In kidney tubule cells the binding of the antagonists to the apical membrane is generally less pronounced than in the intestine. In the renal epithelium the basal membrane is not accessible for nicardipine, but binding of niladipine is observed both to the apical and the basal membrane. The latter result indicates the presence of additional DHPR receptors in the basal membrane. In renal cell suspensions, both antagonists bound to the basal membrane independently from the presence or absence of calcium in the extracellular medium. In contrast to the kidney, only small amounts of both ligands were bound to the apical membrane of the enterocytes. This poor accessibility of the antagonist binding site of the DHPR to the apical membrane was true also for the agonist nitrendipine which elicits calcium-dependent electrical and secretory responses in the intestine. The colocalization of the dihydropyridine receptor antagonist binding sites with the calcium channel on the membrane was demonstrated by immunofluorescent staining of both the intestine and the kidney. The data presented in this paper are consistent with the existence of a second dihydropyridine receptor located on the basal membrane of the intestinal epithelium, which might be essential for transport processes of the epithelium.3D Technology
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Jul 29, 2013. Rachel Steele Milf148 Son S Birthday Present Wmv.
rachel steele milf148 son s birthday present wmv
Merry Christmas Friday, May 15, 2015
If you have followed my blog for the past year, you know that I have recently had my left hip replaced, and the results have not been very good. So much so that doctors have said I may not be able to fully recover.
Luckily, one of my best friends is a Doctor. He happens to be a neurosurgeon, and that means that he is one of the best in the whole world in terms of Neurosurgery. He (Dr. Andrew Bush) has been checking on me constantly and making sure I am doing OK.
As of today, all signs are that I am finally being accepted back into the Neurosurgery community. The